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c-Met and phospho-c-Met in melanoma cells. (a) c-Met expression is detected by immunoblotting in all but one melanoma cell line. Melanocytes are negative. (b) HGF induces phosphorylation of c-Met in the same melanoma cells. Cell lines were treated with HGF at a concentration of 100 ng/mL for seven minutes prior to protein isolation and detection of phospho-c-Met.
