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. 2012 Mar 30;48(2):265–273. doi: 10.1007/s11627-012-9431-6

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© The Author(s) 2012

This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 1. — Protoplast viability. The effect of the age of the explant on viability of protoplasts isolated from leaf mesophyll (A) and hypocotyls (B) of tested cabbage cultivars (±SE). The effect of the genotype (C), age of explant (D), enzyme solution (E), and culture medium (F) on the viability of protoplast isolated from leaf mesophyll of DH lines of cabbage (±SE). Values followed by the same letter are not significantly different (p ≤ 0.05, HSD). Letters with asterisks indicate significant differences in dependency on time of culture, remaining tested factor.