Table 2.
Average effect of the accession, age of explants and culture medium on division frequency (%) of protoplasts isolated from leaf mesophyll of cultivars and DH lines of cabbage (±SE)
| Factor | Time of culture | ||
|---|---|---|---|
| 5 Days | 10 Days | 15 Days | |
| Accession | |||
| Kamienna Głowa | 6.5 ± 0.9 a | 10.2 ± 1.1 a, b | 14.5 ± 1.7 b |
| Amager | 4.0 ± 1.5 a, b, c | 5.7 ± 2.2 b, c, d | 8.3 ± 2.7 b, c, d |
| Kilaxy F1 | 4.9 ± 0.6 a, b | 11.9 ± 2.4 a, b | 16.8 ± 4.0 b |
| Benia F1 | 3.5 ± 1.0 a, b, c | 6.3 ± 1.9 b, c, d | 9.2 ± 2.5 b, c, d |
| Rodeo F1 | 6.2 ± 1.5 a, b | 9.3 ± 2.3 a, b, c | 13.3 ± 3.5 b, c |
| Reball F1 | 7.8 ± 2.0 a | 15.3 ± 2.8 a | 29.8 ± 2.2 a |
| Vertus F1 | 3.3 ± 1.6 a, b, c | 5.3 ± 2.1 b, c, d | 7.0 ± 2.6 b, c, d |
| Line 582 | 0.2 ± 0.1 c | 0.5 ± 0.2 d | 1.1 ± 0.5 d |
| Line 1071 | 1.2 ± 0.4 b, c | 1.8 ± 0.5 c, d | 2.8 ± 0.6 c, d |
| Line 5071 | 2.1 ± 0.5 b, c | 3.8 ± 0.8 c, d | 5.8 ± 1.3 c, d |
| Age of explant | |||
| 2 Weeks old | 1.5 ± 0.3 b | 3.2 ± 1.0 b | 5.6 ± 1.8 b |
| 4 Weeks old | 4.6 ± 0.6 a | 7.6 ± 0.9 a | 11.7 ± 1.5 a |
| 6 Weeks old | 4.3 ± 0.7 a | 8.0 ± 1.3 a | 11.2 ± 2.0 a |
| Mean | 3.7 ± 0.4 a | 6.5 ± 0.7 b | 9.9 ± 1.0 c |
| Culture mediuma | |||
| 8P | 0.6 ± 0.4 b | 1.1 ± 0.7 b | 1.6 ± 0.8 b |
| CPP | 1.7 ± 0.9 a | 3.1 ± 1.3 a | 4.9 ± 2.0 a |
Values in each column followed by the same letter are not significantly different (p ≤ 0.05, Tukey’s honestly significant difference)
aApplied only to DH lines, therefore excluded below mean