Figure 1.

Detection of ssDNA-derived fragments in sequencing libraries. (A) Strategy for sequencing library preparation from single-stranded DNA fragments. (B) The proportion of ITR-containing reads (ITR length 6–20 nt) in α-DMC1, α-RAD51, and two control IgG samples. (C) The distribution of ITR lengths in α-DMC1 and α-RAD51 libraries. Frequency of sequencing read pairs having ITRs of a given length for all fragments (all reads) and type I ssDNA fragments (type I ssDNA) is plotted separately. (D) The length of micro-homology depends on the temperature of the reaction and on the end-repair enzyme.