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. 2012 May;22(5):957–965. doi: 10.1101/gr.130583.111

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© 2012, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 1. — Detection of ssDNA-derived fragments in sequencing libraries. (A) Strategy for sequencing library preparation from single-stranded DNA fragments. (B) The proportion of ITR-containing reads (ITR length 6–20 nt) in α-DMC1, α-RAD51, and two control IgG samples. (C) The distribution of ITR lengths in α-DMC1 and α-RAD51 libraries. Frequency of sequencing read pairs having ITRs of a given length for all fragments (all reads) and type I ssDNA fragments (type I ssDNA) is plotted separately. (D) The length of micro-homology depends on the temperature of the reaction and on the end-repair enzyme.