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. 2012 May;22(5):957–965. doi: 10.1101/gr.130583.111

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© 2012, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — Enrichment of ssDNA-derived fragments in sequencing libraries. (A) Kinetic enrichment increases the proportion of ssDNA-derived reads in the sequencing libraries. The percentage of type I ssDNA- (ssDNA) and dsDNA-derived (dsDNA) reads in sequencing libraries prepared with (KE) or without (standard) kinetic enrichment. Antibody used in ChIP (α-DMC1 or α-RAD51) and genotypes (Psmc3ip^−/− or wt) of the mice used are indicated on the graph. (B) The distribution of ITR lengths in type I ssDNA fragments from two KE libraries. (C) Asymmetric sequencing largely corrects bias against longer ITRs. ITR length distribution for read pairs where the first read is uniquely mapped and the second read is not mapped using either 36 nt (36/36) or 40 nt (36/40) long second reads is plotted on the graph.

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