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. 2012 Apr 15;26(8):846–856. doi: 10.1101/gad.180679.111

Figure 1.

Figure 1.

rde-10 and rde-11 are required for RNAi and transgene silencing. (A) Schematic representation of the vha-6p::atgl-1b::gfp transgene (hjIs21). Images of larval stage L1 or L4 wild-type animals carrying hjIs21 are shown, together with rde-1(ne300), rde-10(hj20), rde-11(hj37), rde-10(hj20);hjSi10[rde-10(+)], and rde-11(hj37);hjSi116[rde-11(+)] L4 mutant animals carrying the same transgene. Bar, 100 μm. (B) Quantitation of atgl-1::gfp mRNA level by real-time PCR. The mRNA level in wild-type L1 animals was set as 1. Mean + standard deviation from triplicate reactions are shown. (C) Molting defect after nhr-23 RNAi in wild-type, rde-10(hj20), rde-11(hj37), rde-10(hj20);hjSi10[rde-10(+)], rde-11(hj37);hjSi116[rde-11(+)], rde-11(hj37);hjEx11[rde-11(+)], and rde-11(hj37);hjEx12[rde-11(C203S)]. Mean + standard deviation from three independent experiments are shown.