Table 3. Characteristics of sorted ascospores.

Parameter Tested	Sorted Ascospores
	RFP−	RFP+
Physical counta	6.7 × 106	6.3 × 106
Viable countb	4.2 × 106	4.5 × 106
Viability, %	63	71
Contamination with HygR cells, %c	0.16	N/A
Contamination with diploid cells, %d	N/A	0.21

^aCounted in a hemocytometer.

^bSerial dilutions were spread onto YPD plates and colonies were counted after 2 days at 30°.

^cRFP-negative cells (2 × 10³ CFU/plate) were spread onto YPD plates containing 200 µg/mL of hygromycin-B. Colonies were counted after 3 days at 30°.

^dRFP-positive cells (20−50 CFU/plate) were grown on YPD for 2 days at 30° and replica-plated onto lawns of a MATα tester strain. After 2 days at 30°, the colonies were scored for halo formation. Diploids were implicated by lack of halo formation.