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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2753–2765. doi: 10.4049/jimmunol.0801124

FIGURE 1.

FIGURE 1

Treg levels are increased in LLC-bearing lung tissue. Immunofluorescent staining was performed on frozen sections from normal lung tissue (A) and LLC-bearing lung tissue (B) for nuclei (Hoechst, blue), CD4 (rhodamine, red) and FoxP3 (FITC, green). Tregs (white arrows) are considered CD4+ cells that also contain FoxP3 staining in the nucleus (merge). Tumor periphery is marked with a dashed white line. Insets are of a higher magnification of the indicated field. Immunofluorescent staining of CD4, CD25, and FoxP3 was also preformed on normal and LLC-bearing lung tissue dissociates. Flow cytometric analysis shows an increase in total Treg numbers (C) and an increase in the Treg percentage of CD4 infiltrate (D) in LLC-bearing lung tissue. Tregs that were immunomagnetically isolated from both normal and LLC-bearing lung tissue displayed the ability to suppress the proliferation of endogenous CD4+CD25− cells isolated from the respective tissue types (E).