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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2753–2765. doi: 10.4049/jimmunol.0801124

FIGURE 4.

FIGURE 4

CCL22 mediates selective recruitment of Tregs toward conditioned medium. Whole T cells were isolated from normal splenocytes and used in Transwell migration assays to measure selective Treg recruitment toward normal (light bars) and LLC-bearing lung (dark bars)-conditioned medium with (striped bars) or without (solid bars) neutralizing Ab against CCL22. Following the assay, the Treg percentage of CD4+ cells was measured using flow cytometry in both the migratory fractions in the bottom chamber (A) and the nonmigratory fractions left in the top chambers (B). Inhibition of selective Treg recruitment using neutralizing Ab against CCL22 in a Transwell migration assay is dose dependent in both normal and LLC-bearing lung-conditioned medium and Ab specific (C).