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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: Curr HIV Res. 2011 Jul;9(5):276–288. doi: 10.2174/157016211797635928

Fig. 3.

Fig. 3

Heteroduplex Mobility Assay (HMA). Viral diversity in macaques administered low or high doses of SIV. Genomic DNA was obtained from PBMCs from the first SIV-positive sample of all orally inoculated macaques (day 14 for low dose macaques, and day 7 or 14 for high dose macaques) and the V1-V2 region of SIVenv was PCR amplified. Lanes 1 to 6 show the six macaques that were inoculated with low doses of SIVmac251 (lane 1: 17742, 2: 18984, 3: 18412, 4: 18414, 5: 18981, 6: 19147). Lanes 7 to 11 show the five macaques inoculated with high doses of SIVmac251 (lane 7: RM11, 8: RM12, 9: RM13, 10: RM14, 11: RM15). Lane 12 shows HMA for V1-V2 region of a SIVmac239 plasmid, to indicate the pattern when only one clonal sequence is present. HD indicates the homoduplex bands which is the brightest and fastest migrating band on the gel.