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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Immunol. 2012 Mar 7;188(8):3611–3619. doi: 10.4049/jimmunol.1102475

Figure 2. Over expression of TXK rescues α-GalCer induced cytokine secretion of iNKT cells in the absence of ITK.

Figure 2

WT, Itk−/− and Tg(CD2-Txk)Itk−/− mice were injected intraperitoneally with either PBS or 2 μg α-GalCer. After 2 hours, splenocytes were collected and cultured in-vitro for 4 h in the presence of 10 μg/ml Brefeldin A. Cells were analyzed by flow cytometry, gated on iNKT cells (tetramer+/TcRβ+) and analyzed for IL-4 and IFN-γ expression. PBS control (shaded), α-GalCer (solid line). Data are representative of two independent experiments.