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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Biochemistry. 2012 Mar 1;51(10):2146–2156. doi: 10.1021/bi300097g

Figure 3.

Figure 3

a) Aminoacylation of tRNAPro with proline by WT, G217A, and E218A Ec ProRS. The assay was performed at 37 °C with 0.5 µM tRNAPro and 100 nM Ec ProRS. b) Pre-transfer editing in the presence of alanine by WT, G217A, E218A Ec ProRS. The assay was performed at 37 °C using 0.5 µM ProRS and 500 mM alanine. Lines are single exponential fits of the data.