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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: Cancer Lett. 2010 Oct 25;300(1):66–78. doi: 10.1016/j.canlet.2010.09.007

Figure 2. Correlations of protein levels with ZEB1 in NSCLC.

Figure 2

For each cell line, 10 μg total protein were loaded on a 4–15% polyacrylamide gel. Cell lines were ranked on the gel according to ZEB1 mRNA from low to high levels (left to right). Western blots were performed with indicated antibodies. Molecular weights are provided in kDa. β-actin was used as a loading control. Among these cell lines, those with known mutations of EGFR and sensitivity to an EGFR inhibitor include H3255, HCC4006, H1650 and HCC2279 [48]. In addition, those with IC50s of 1 μM or less in a proliferation assay include H358 and H322 [48].