Reactive oxygen species (ROS) production in isolated mitochondria with succinate (Succ) as substrate. The effects of antimycin-A (A) and ubiquinone (B) on complex II-linked ROS production in the presence or absence (Con) of isoflurane (Iso) were analyzed after reverse electron flow induced-ROS was blocked by rotenone. Iso reduced reverse electron flow induced-ROS production in the absence of rotenone, however, Iso had no effect in the presence of rotenone, and antimycin A or ubiquinone. (C) Summary of recordings. Data are presented as mean±SD, n=8/group, *P<0.05 versus corresponding Con, #P<0.05 versus the respective Con with Succ alone, §P<0.05 versus respective Con with rotenone.