Figure 2.

Biolumienscent Akt reporter (BAR). A. The domain structure (upper) and the proposed mechanism of action (lower) of the BAR reporter. It involves Akt-dependent phosphorylation of the Akt-pep domain (thick line), which results in its interaction with the FHA2 domain (right). In this form, the reporter has minimal bioluminescence activity. In the absence of Akt activity, the N-Luc and C-Luc domains reassociate, restoring bioluminescence activity (left). B. Bioluminescence imaging of Akt kinase activity. Bioluminescence activity before treatment (time 0) and in response to treatment with API-2 (20 mg/kg or 40 mg/kg) was monitored at various times. Reproduced with permission from ref. 61.