Figure 3.
Treatment with ATP (100 µmol/L, 5 minutes) altered whole endothelial cell endothelial NO synthase (eNOS) multisite phosphorylation. A, Total cav-1, total eNOS, and GAPDH were not different between control ■ and ATP ▩ groups. B, Stimulatory PSer635eNOS was significantly increased (*) in response to ATP vs the control group. Stimulatory PSer1179eNOS was unaltered. The inhibitory PThr495eNOS was significantly decreased in response to ATP. The agonist-specific PSer114eNOS was significantly increased in response to ATP treatment.