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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Neurosci. 2012 Mar 11;15(5):703–712. doi: 10.1038/nn.3070

Figure 5. Nogo receptors mediate CSPG inhibition.

Figure 5

(a) In vitro, WT P7 CGNs are strongly inhibited when plated on substrate–bound CSPGs (10μg/ml). Studies with CGNs from NgR1−/−, NgR12−/−, and NgR3−/− mutants revealed no significant release of CSPG inhibition. Loss of all three NgRs (NgR123−/−) or RPTPσ alone (RPTPσ−/−) leads to a significant, yet incomplete release of CSPG inhibition. On a control substrate (BSA), neurite length of all six genotypes is comparable. (b) Quantification of neurite length. Gray bars (BSA); black bars (CSPGs). At least 300 neurites of TuJ1–labeled cells were counted per condition (n=8 independent experiments). Results are presented as mean ±SEMs. ** P< 0.001 (one way ANOVA, Tukey’s post–hoc), n.s.= not significant. Scale bar, 70μm.