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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Oncogene. 2012 Jan 23;31(46):4835–4847. doi: 10.1038/onc.2011.648

Figure 4. Effect of endogenous angiogenin silencing on the expression of p53 target genes and pro-survival Bcl-2 gene.

Figure 4

(A–D) cDNA prepared from 293T cells transfected with si-ang or si-RL (renilla luciferase) were used to measure p53 (A) and p53 target genes p21 (B), Bax (C) and Bcl-2 (D) expression by quantitative real-time PCR. Untransfected cells were used as control. For all the above, each reaction was done in triplicate and each bar represents the mean ± SD from three independent experiments. (**) p<0.001, (***) p<0.0001 compared with the control. (E) Western blot analysis showing p53 target gene p21, Bax and Bcl-2 expression in 293T cells transfected with si-RL or si-ang. Actin was used as loading control.