Fig. 1.

Exercise-induced activation of the mitochondrial biogenesis pathway in skeletal muscle. Western blotting analysis to detect PGC-1α (a and d), NRF-1 (b and e), and cytochrome C (c and f) in PGC-1α KO mice and aged rats. Twenty-seven male mice were randomly divided into four experimental groups: WT untrained (n = 6), WT trained (n = 8), KO untrained (n = 7), and KO trained (n = 6). Twenty-four male Wistar rats were randomly divided into four experimental groups: young untrained (n = 6), young trained (n = 6), aged untrained (n = 6), and aged trained (n = 6). Representative blots are shown. For the densitometric analysis of the results, values are shown as mean (±SD).The content of α-actin, a housekeeping protein marker in skeletal muscle, was determined in all the experimental groups