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. Author manuscript; available in PMC: 2013 May 8.
Published in final edited form as: Oncogene. 2012 Jan 23;31(45):4750–4758. doi: 10.1038/onc.2011.633

Figure 5. Inhibiting CXCR7 reduces growth of CXCR4+ breast cancer cells in orthotopic tumors.

Figure 5

Mice were implanted with orthotopic breast tumor xenografts comprised of human mammary fibroblasts expressing CXCL12-GL (HMF-CXCL12-GL), 231-CXCR4-FL cells, and 231-CXCR7 cells (n = 10 per group). After palpable tumors developed one week after implantation, mice were treated daily with subcutaneous injections of CXCR7 inhibitor CCX771 (30 mg/kg) or vehicle control. A) CXCL12-GL in serum samples was quantified after one week of treatment. Data are presented as mean values + SEM. B) Quantified firefly luciferase bioluminescence from 231-CXCR4-FL cells in primary tumors after 4 weeks of tumor growth. Graph shows mean values + SEM. C) Representative firefly luciferase bioluminescence images of 231-CXCR4-FL metastases in mice treated with CCX771 or vehicle control. Scale bar depicts range of values for photon flux as a pseudocolor display. D) Quantified data for photon flux from 231-CXCR4-FL metastases in multiple anatomic sites. Graph displays mean values + SEM. *, p < 0.05.