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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Vet Microbiol. 2011 Dec 14;157(1-2):23–31. doi: 10.1016/j.vetmic.2011.12.008

Fig. 4. Detergent effects are different in distinct TSE.

Fig. 4

Homogenates of CWD in elk or atypical/Nor98 scrapie were prepared using different detergents. A) CWDelk: 1 % SDS, 2 % SLS and 1 % DOC enhance the PrPSc signal while 5 % SLS slightly reduces it compared to digestion without detergents. B) Atypical/Nor98 scrapie: Preparation in PBS results in the strongest PrPSc signal and all detergents tested on this blot reduce it. Proteinase K in the presence of the mild detergent DOC degrades PrPSc of atypical/Nor98 scrapie more than 2 % SLS or 1 % SDS do (mAb P4 1:2000).