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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Neurol Res. 2011 Dec;33(10):1083–1093. doi: 10.1179/1743132811Y.0000000053

Figure 2.

Figure 2

Micrographs (×20) showing the differentiation of neural stem cell (NSC)-like cells in culture medium containing neurobasal and B27 without basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). NSC-like cells differentiated into cells expressing beta-III-tubulin (a and b, green) and glial fibrillary acidic protein (GFAP) (A and B, red), respectively. A subpopulation of differentiated neuronal cells expressed neurofilament 200 (NF-200) (C, green), gamma aminobutyric acid (GABA) (D, red), glutamate (E, green), and microtubule-associated protein 2 (MAP2) (F, green), respectively. Some NSC-like cells differentiated into cells expressing nerve/glial antigen 2 (NG2) (G, green), a marker for oligodendrocyte progenitors. Some NSC-like cells differentiated into cells expressing galactosylceramidase (GalC) (H, red), a marker for oligodendrocytes. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI) (blue).