A representative picture (×20) with triple fluorescent immunocytochemical staining for neuronal differentiation of the neural stem cell (NSC)-like cells in the culture medium consisting of neurobasal and B27 without basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). NSC-like cells differentiated into neuronal cells expressing doublecortin (DCX) (A, blue), beta-III-tubulin (B, red), and microtubule-associated protein 2 (MAP2) (C, green). Note that some cells double labeled with different markers may represent the neuronal cells at different stages of differentiation (D, yellow). Counterstaining for cell nuclei was not performed in these cells.