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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Neurol Res. 2011 Dec;33(10):1083–1093. doi: 10.1179/1743132811Y.0000000053

Figure 3.

Figure 3

A representative picture (×20) with triple fluorescent immunocytochemical staining for neuronal differentiation of the neural stem cell (NSC)-like cells in the culture medium consisting of neurobasal and B27 without basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). NSC-like cells differentiated into neuronal cells expressing doublecortin (DCX) (A, blue), beta-III-tubulin (B, red), and microtubule-associated protein 2 (MAP2) (C, green). Note that some cells double labeled with different markers may represent the neuronal cells at different stages of differentiation (D, yellow). Counterstaining for cell nuclei was not performed in these cells.