Typical fluorescent immunohistochemical pictures (×20) in the mouse brain 4 weeks after neural stem cell (NSC)-like cell transplantation. NSC-like cells survived, migrated, and differentiated into astrocytes expressing glial fibrillary acidic protein (GFAP) (A), neuronal cells expressing beta-III-tubulin (B), and neuronal nuclei (NeuN) (C). Colocalization of bromodeoxyuridine (BrdU) (red) with GFAP or beta-III-tubulin or NeuN (green) immunoreactivity indicated that these cells originated from transplanted NSC-like cells. The yellow color in the pictures is produced by an overlap of BrdU (red) and beta-III-tubulin or NeuN (green) staining. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI) (blue).