FIG. 2.

Effect of pretreatment with TFA for 24 weeks on glomerular cells apoptosis in DN rats by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay (n=6 per group): (A) control group, (B) DN group, (C) LTFA group, and (D) HTFA group. ×400 magnification. Positive glomerular cells in formalin-fixed renal slices were determined by examining at least 30 glomeruli in each rat. (E) The bar graph represents the mean percentages of apoptotic cells. Data are mean±SD values. P<.05 is statistically significant. ^aSignificantly different compared with the control group. ^bSignificantly different compared with the DN group. Color images available online at www.liebertonline.com/jmf