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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Proteomics Clin Appl. 2012 Apr;6(3-4):201–211. doi: 10.1002/prca.201100068

Figure 1. Membrane enrichment strategy.

Figure 1

(A) Flowchart of experimental design to enrich for membrane proteins from human brain tissue. (B) Silver stain showing differential protein banding patterns in each fraction. Immunoblot of PSD-95, a transmembrane domain-containing protein specific to the post synaptic density, demonstrates approximately 2 fold enrichment in the membrane fraction compared with total homogenate. (C) Immunoblots for calnexin, synaptophysin (SYP) and SSBP1 demonstrates that multiple membrane proteins are present in the final membrane fraction.