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. Author manuscript; available in PMC: 2012 Apr 27.
Published in final edited form as: Science. 2011 May 13;332(6031):811–816. doi: 10.1126/science.1203983

Figure 1. Expanding colonies are generated from isolated smedwi-1+ cells following irradiation.

Figure 1

(A–B) Proliferating cells were detected by smedwi-1 expression using whole-mount in situ hybridization (ISH). Anterior, up. Ventral surface shown. (B) Representative images 7 days after 1,750-rad treatment show clusters (arrowheads) of smedwi-1+ cells (individual purple dots). (C) Histogram of cluster frequencies following 1,750 rads. (D) Clusters observed by smedwi-1 ISH 7 days post-1,750 rads displayed in a scatterplot. phx, pharynx. (E–F) Animals fixed in a timecourse after 1,750-rad treatment analyzed by smedwi-1 fluorescence in situ hybridization (FISH). (F) Mean cluster frequency (#clusters/worm) and size (#smedwi-1+ cells/cluster) are plotted. Error bars, standard deviation (n=17–22 animals/timepoint). (G) IF (BrdU) and FISH (smedwi-1). 234/234 BrdU+ cells (8-hour BrdU-pulse in seven-day-irradiated worms) were smedwi-1+. (H) IF (SMEDWI-1) and FISH (smedwi-1); 12/12 colonies contained SMEDWI-1+; smedwi-1− cells (arrowheads) 7 days post-1,750 rads. (I) IF (BrdU) and FISH (smedwi-1). 31/31 colonies (with BrdU pulse days 7–11 post-1,750 rads) contained BrdU+; smedwi-1− cells. Scale bars, 200µm (A–B), 20µm (E, G–I).