Figure 1 .

Creation and phenotypic characterization of a ΔFWO strain. (A) Crossing strategy used to generate a ΔFWO strain. All strains carry the ras-1^bd mutation (not shown). (B) Extension rates of indicated strains in LL (open bars) and DD (shaded bars) at 25°. Error bars indicate ±1 SD. (C and D) Representative race-tube images from strains grown in DD and LL at 25°. (E and F). Race-tube images of wild-type (wt) and ΔFWO strains grown in the indicated temperature skeleton T cycles. The duration of each 28° temperature pulse was 2 hr. Shaded bars: 22°C; open bars: 28°.