Figure 6 .

Temperature-dependent effects on viability of a cdc13-S611L rad9-Δ strain. (A) Mid-log cultures of cdc13-S611L (Left) or cdc13-S611L rad9-Δ (Right, duplicate samples) strains grown at 23° were shifted to the indicated temperatures and incubated for up to 8 hr; viability was determined by plating appropriate dilutions on rich media plates that were incubated at 23° for 3 days. Three independent repetitions of this experiment produced essentially identical results. (B) Flow cytometry profile of log-phase cultures of the indicated strains from the experiment shown in A, fixed and stained with SYTOX green.