Figure 3.

Cyst formation and signaling in iKsp-Pkd1^del mice. Hematoxylin and eosin staining of renal sections from iKsp-Pkd1^del mice at (A through C) 80 days or (D through F) 105 days of treatment. A and D show mice receiving no treatment, B and E show mice receiving low-dose sirolimus, and C and F show mice receiving high-dose sirolimus. Renal sections stained with p-S6Rp^Ser240/244 (G through L, P), p-Akt^ser473 (M through O, Q), or p-ERK1/2^{Thr202/Tyr204} (R). (M and N) Arrowheads indicate more or less intense p-Akt^ser473 signal (black and white arrowhead, respectively). (P through R) Dilated tubules 1–3 are positive for p-S6Rp^Ser240/244 but negative for p-Akt^ser473 and p-p90 RSK^{Thr359/Ser363} arrowheads indicate tubules negative for p-S6Rp^Ser240/244, positive for p-Akt^ser473, and with most of the cells negative for p-p90 RSK^{Thr359/Ser363}.