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. 2012 May;23(5):814–824. doi: 10.1681/ASN.2011060567

Figure 4.

Figure 4.

miR-148b targets C1GALT1. (A) Genomic localization of miR-148b in chromosome 12q13. (B) Sequence alignment of the miR-148b base-pairing sites in the 3′-UTR of C1GALT1 mRNA showing that the regions complementary to miR-148b are highly conserved among human, chimp, mouse, rat, dog, and chicken. The “seed” sequences of miR-148b complementary to C1GALT1 are shown in gray. (C) Linear correlation between the expression of C1GALT1 and the expression of miR-148b in PBMCs of 10 patients with IgAN and 10 healthy subjects (HSs). C1GALT1 mRNA levels inversely correlated with miR-148b expression levels (R2=0.4; P<0.01). (D) Output of bioinformatic analysis performed by MicroSNiPer algorithms showing that the 1365G/A polymorphism (rs1047763) affects miR-148b binding sites in the 3′-UTR of C1GALT1. The 1365G allele (arrow) enhances miR-148b binding, whereas the 1365A allele does not appear in the output as a possible binding site for miR-148b.