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. 2012 May;23(5):814–824. doi: 10.1681/ASN.2011060567

Figure 6.

Figure 6.

miR-148b regulates C1GALT1 mRNA expression. (A) C1GALT1 expression levels were analyzed by real-time PCR in PBMCs of healthy subjects (HSs) after transfection with miR-148b mimic. Increasing the amount of miR-148b within the PBMCs of HSs resulted in a three-fold reduction in endogenous C1GALT1 mRNA levels. (B) C1GALT1 expression levels were analyzed by real-time PCR in PBMCs of five other HSs after transfection with the mutated miR-148b mimic, Δ20-21, and miR-148b mimic wild-type. C1GALT1 mRNA expression decreased only after transfection with the wild-type miR-148b mimic. (C) C1GALT1 expression levels were analyzed by real-time PCR in PBMCs of patients with IgAN after transfection with miR-148b inhibitor. Silencing the activity of miR-148b within IgAN PBMCs led to a three-fold increase in endogenous C1GALT1 mRNA levels. “Mock” indicates mock-transfected cells going through the transfection processes without addition of mimic/inhibitor miRNA. Expression data were normalized on the housekeeping gene β-actin. Data are representative of four independent experiments for A and C and five independent experiments for B (mean ± SEM). **P<0.01.