Table 2.
Validation Panel Reproducibility Specimens and Amplification Results
| Amplification Results (No. of Positive/No. of Replicates) |
||||||||
| Spec ID | Subtype | Viral Load, copies/mL | PR RAMs | RT RAMs | Mixed Bases, %a | Min | Max | No. of laboratories ≥80% |
| VP1 | CRF02 | 143 475 | L10V, L33V, L89M | None | 1.0 | 3/5 | 5/5 | 9/10 |
| VP2 | G | 105 264 | V82I, L89M | K103N | 0.5 | 4/5 | 5/5 | 10/10 |
| VP3 | B | 5588 | L10I | Y181C | 1.6 | 4/5 | 5/5 | 10/10 |
| VP4 | CRF02 | 158 858 | L89M | None | 3.7 | 5/5 | 5/5 | 9/9b |
| VP5 | C | 10 927 | L89M | None | 2.8 | 4/5 | 5/5 | 9/9 |
| VP6 | B | 1812 | None | M41L, D67N, L74I, V90I, K103N, Y181C, M184I/V, L210W, T215Y | 0.05 | 0/5 | 5/5 | 1/9 |
Abbreviations: PR, protease; RAMs, resistance-associated mutations (as defined by the IAS-USA [8]); RT, reverse transcriptase.
a
Percentage of base calls other than A, C, G, or T in the consensus sequence from all laboratories.
b
One laboratory did not generate results for VP4-VP6 because of a processing error.