Table 3.

Distribution of Errors at Drug Resistance Mutation Sites, Compared With Sequence Heterogeneity

		Base Composition				Mixtures at DRM Sites			Errors at DRM Sites
Panel	Specimen	No. Mixed	No. Unmixed	% Mixed	% Mixed, no Nsa	PR	RT	Total	PR	RT	Total
1	1	9	870	1.02%	0.11%	1	0	1	10	1	11
	2	1	878	0.11%	0.11%	0	0	0	0	0	0
	3	1	878	0.11%	0.11%	0	0	0	0	0	0
	4	18	861	2.05%	0.92%	2	0	2	9	0	9
	5	17	883	1.89%	1.12%	3	0	3	11	0	11
2	1	6	873	0.68%	0.00%	0	0	0	1	4	5
	2	8	871	0.91%	0.80%	2	0	2	14	8	22
	3	0	879	0.00%	0.00%	0	0	0	0	4	4
	4	6	873	0.68%	0.23%	2	0	2	14	12	26
	5	1	878	0.11%	0.00%	0	0	0	3	4	7
3	1	0	879	0.00%	0.00%	0	0	0	0	2	2
	2	16	863	1.80%	0.90%	3	0	3	24	2	26
	3	1	878	0.10%	0.00%	0	0	0	2	12	14
	4	2	877	0.20%	0.00%	0	0	0	16	13	29b
	5	15	864	1.70%	0.80%	1	0	1	3	22	25
4	1	27	852	3.07%	1.50%	2	1	3	13	0	13
	2	0	879	0.00%	0.00%	0	0	0	9	9	18
	3	18	861	2.05%	0.81%	3	2	5	26	19	45
	4	19	860	2.16%	2.16%	0	5	5	8	49	57
	5	25	854	2.84%	0.81%	4	2	6	24	13	37

Abbreviations: DRM, drug resistance mutation; PR, protease; RT, reverse transcriptase.

^aNot counting Ns because these are ignored in scoring.

^bTwenty-four of these discrepancies were a result of the wrong sequence being submitted (possible contamination).