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Extracted ion chromatography of tetrasaccharide a, b and c on treatment with WT and mutant HepII by LC-MS analysis. A. tetrasaccharide a; B. tetrasaccharide b; C. tetrasaccharide c. The four panels shown in each set of chromatograms (from top to bottom) correspond to untreated tetrasaccharide, WT treated tetrasaccharide, N405A tetrasaccharide, and N405G tetrasaccharide. Fractions from each treatment are listed in Table 1.
