Figure 3.

Representative flow cytometry experiment (top) using cells incubated for 24 hours and then labeled with annexin V conjugated to Alexa Fluor 488 and MitoTracker Red before study. Apoptosis increased after exposure of HK-2 cells to the two monoclonal FLCs, but cells pretreated with siRNA that targeted ASK1 were protected from FLCs-induced apoptosis. The graph at the bottom represents a compilation of 8 to 10 experiments in each group. *P < 0.05 compared to siRNA alone and siRNA with λ3 and κ2.