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. 2012 Jan;14(1):22–29. doi: 10.1016/j.jmoldx.2011.08.002

Figure 3.

Figure 3

Single RNA molecule detection. A: HER2 genomic DNA in HeLa and SK-BR-3 cells was detected using the RNAscope probes and signal amplification system. Nuclei were costained for IL-8 for diploid genome Original magnification, ×40. B: HER2 mRNA detection in HeLa cells using the same probes and signal amplification system. A probe set to 18S rRNA was used as internal control for RNA detection. HER2 mRNA dots were counted; mean dots per cell (±95% confidence limit) are indicated. Nuclei were counterstained with DAPI (blue). Original magnification, ×40. C: HER2 mRNA copies per cell in HeLa cells determined by QuantiGene 2.0 (QG2) using a standard curve from in vitro transcribed RNA. Mean copies per cell (±95% confidence limit) are indicated (green dot), as calculated from triplicate measurements using linear regression.