Figure 4.

Absence of ubiquitin-positive inclusions and cell death in TgTDP-25 mice. A: Representative hippocampal sections from 6-month-old NonTg and TgTDP-25(B) mice stained with a phospho-specific TDP-43 antibody (n = 6/genotype). B: Cortical sections from 6-month-old TgTDP-25(B) mice were double stained with a C-terminal anti-TDP-43 antibody and with an anti-ubiquitin antibody. The overlay shows that at this age, the TgTDP-25(B) mice have no ubiquitin-positive TDP-43 inclusions. C: Representative cortical and hippocampal sections from 6-month-old NonTg and TgTDP-25(B) mice stained with Fluoro-Jade. D: Quantitative analysis of the Fluoro-Jade–positive cells, conducted as described in Materials and Methods, showed no statistically significant difference between the two groups of mice (n = 6/genotype). Data are presented as means ± SEM and analyzed by Student's t-test.