Figure 7. Control SPR experiments to map the binding segment of SNX1.

(A) Injection of equivalent 15 µM amounts of SNX-PX and PX domains are superimposed. A CM5 chip coupled with RPRdel was used in these experiments. (B) Injection of 30 µM SNX-PX protein onto a CM5 chip coupled with Rab6/RPdel. Note that the magnitude of the binding, as evidenced by the y-axis (RU), is 10-fold smaller. These representative control experiments show that SNX-PX binds stably to DENND5-coupled chips that include the RUN2 domain. Data from the control experiments could not be processed and fit to conventional kinetics or equilibrium binding models using the evaluation software.