Figure 5. Effects of pressure overload on the myocardial expressions of extracellular matrix and sarcomeric remodeling-related elements.
mRNA relative expressions of A: Collagen I (Col I); B: Collagen III (Col III); C: Fibronectin 1 (FN); E: α-Myosin heavy chain (α-MHC); F: β-Myosin heavy chain (β-MHC) in female and male mice under the following experimental conditions: Sham operated, castrated (CAST), subjected to aortic arch constriction (TAC), and castrated subjected to TAC (CAST+TAC). Data are expressed as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 (ANOVA and Bonferroni post-hoc test). D: Representative histological sections illustrating myocardial tissue fibrosis (polarized light, picrosirius red stain, original magnification: 50×).