Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 1;23(9):1609–1617. doi: 10.1091/mbc.E11-06-0536

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Mallory et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — In vivo Ume6p acetylation requires Gcn5p. (A) In vitro acetylation assays described in Figure 1 were repeated with the indicated Gst-Ume6 substitution mutants as substrate. Gels were Coomassie stained (Coom.) before fluorography and used to control for substrate addition. (B) Extracts prepared from log-phase RSY10 cultures expressing the indicated GAL-T7-UME6 alleles (or the vector control) were blotted and probed with α-K736-Ac antibodies. (C) The experiment described in B was repeated in the wild-type (RSY10) and gcn5∆ mutant (RSY622) strains. (D) Endogenous Ume6p (RSY1079) or Ume6p^KallR (RSY1149) was detected in extracts derived from mid-log cultures growing in dextrose, acetate, or after the switch from acetate to SPM. K736 acetylation was monitored by Western blot analysis probing with α-K736-Ac. Ponceau staining of the gel is shown as loading control.