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. 2012 May 1;23(9):1609–1617. doi: 10.1091/mbc.E11-06-0536

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© 2012 Mallory et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Ume6p acetylation is required for normal meiotic gene expression and progression. (A) Northern blot analysis of early (SPO13), middle (SPS4), and late (SPS100) meiotic mRNAs in either UME6 (RSY1079)-expressing or UME6^KallR (RSY1149)-expressing stains after transfer to SPM as indicated. Western blot analysis was used to monitor Ume6p and Ume6p^KallR levels (bottom) at the same time points. (B) Wild-type (RSY1079, open circles) and Ume6p^KallR (RSY1149)-expressing (open squares) and Ume6p^KallQ (RSY1226)-expressing (closed diamonds) strains were induced to enter meiosis and time points taken as indicated. The percentages of cells containing multiple nuclei indicating the execution of at least one meiotic nuclear division were determined by DAPI staining.