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. 2012 May 1;23(9):1618–1627. doi: 10.1091/mbc.E11-11-0933

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© 2012 Rao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Inhibition of Tom40 import by PKA is not connected to the phosphorylation of Tom70 by PKA. ³⁵S-Labeled precursor of Tom40 was imported into mitochondria, which were isolated from Tom70^S174E, Tom70^WT, or Tom70^S174A yeast strains, at 25°C in the presence or absence of PKA (New England BioLabs) as indicated. The mitochondria were solubilized in digitonin-containing buffer and analyzed by blue native electrophoresis and digital autoradiography.