FIGURE 5:

IGPR-1 regulates focal adhesion and inhibits paxillin phosphorylation and cell migration. PAE cells expressing either empty vector or IGPR-1 were grown in 10% fetal bovine serum/DMEM medium and subjected to immunofluorescence staining as described in Materials and Methods. The immunofluorescence staining (green is vinculin staining, purple is nuclear staining, and red is phalloidin staining for F-actin) of PAE cells expressing empty vector vs. IGPR-1 is shown (A vs. D, B vs. E, C vs. F). (G) Quantification of focal adhesion PAE cells expressing empty vector, pMSCV, or IGPR-1. Cell lysates derived from PAE cells expressing empty vector and IGPR-1 were immunoblotted with anti–phospho-Y118 paxillin (H), anti–IGPR-1 (I), and IQGAP1 for protein loading control (J). (K, H) PAE and B16F cells expressing empty vector vs. IGPR-1 were subjected to wounding assay, and migration of cells toward the wound area was documented as described in Materials and Methods.