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. 2012 May 1;23(9):1646–1656. doi: 10.1091/mbc.E11-11-0934

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© 2012 Rahimi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License(http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — SPIN90 activity regulates angiogenesis. PAE cells induced to express IGPR-1 alone or coexpress IGPR-1 with SPIN90. (A) Cells were subjected to Matrigel-based angiogenesis assay, and capillary tube formation was documented after 48 h. (B) Quantification of capillary tube formation determined using ImageJ software. (C, D) Whole-cell lysates derived from these cells blotted for IGPR-1 and SPIN90. (E) HUVECs were transfected with control siRNA or SPIN90-siRNA, and after 46 h, cells were subjected to Matrigel-based capillary tube formation, and pictures were taken after 48 h. (F) Quantification of capillary tube formation. (G) Expression of SPIN90 and protein loading control, PLCγ1, in HUVECs.