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. 2012 May 1;23(9):1664–1674. doi: 10.1091/mbc.E11-12-0988

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© 2012 Mashukova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — PDK1 distributes to a subapical vesicular compartment and the apical plasma membrane in Caco-2 cells and in intestinal crypts. Cultures were processed for immunogold with (A) nonimmune IgG or (B, C) anti-PDK1 antibody, silver enhanced, and viewed under a TEM. Arrows point at apical membrane-associated gold particles. (D, E) Frozen sections of mouse duodenum were processed with (D) nonimmune IgG, or (E) anti-PDK1 antibody (red channel) for immunofluorescence and counterstained with DAPI (blue channel). (F) Confocal xz-sections of lentiviral PDK1-knockdown Caco-2 cells (shRNA) or mock-transduced cells processed with the same anti-PDK1 antibody (red channel). Bars, A, 1.4 μm; B, 1.9 μm; C, 0.4 μm; D and E, 20 μm; F, 10 μm.