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. 2012 Apr 25;7(4):e35960. doi: 10.1371/journal.pone.0035960

Figure 4. CC10 gene transfer prevents NF-κB nuclear translocation in BEAS-2B cells.

Figure 4

BEAS-2B cells transfected with either mock or pCC10 were treated with 10 ng/mL of IL-1β for 30 or 60 minutes. Total and nuclear proteins were extracted and then performed to western blot. (A) Nuclear NF-κB p65 (N-p65) and (B) total p65 (T-p65) were detected. One representative experiment of the three independent experiments is shown. (C) and (D) The semi-quantitative analysis of western-blot gels and results were presented as relative protein expression levels. n = 3, ** P<0.01 and * P<0.05. CC10, Clara cell 10-kDa protein; IL, interleukin.