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. 2012 Apr 25;7(4):e35960. doi: 10.1371/journal.pone.0035960

Figure 6. CC10 does not interact with NF-κB p65/p50 subunits directly.

Figure 6

BEAS-2B cells transfected with pCC10 were stimulated with 10 ng/mL of IL-1β for 4 hours. Cellular proteins were immunoprecipitated with anti-p65 antibody. Immunoblotting was performed with anti-p65 and anti-CC10 antibodies (lane 1). Rabbit normal serum was served as a negative control for immunoprecipitation (lane 2) and human inferior turbinate mucosa were used as positive controls for CC10 and p65 expression (lane 3). One representative experiment of the three independent experiments is shown.