Figure 8. LAURDAN GP image of sedimented material from C₁₂SM LUVs treated with SMD.

A) Sedimented control 100 nm liposomes after 24 hours (without SMD) at the bottom of the slide. Average GP value is −0.029±0.129. B) Sedimented material of LUVs treated with 1 µg/ml SMD after 24 hours at the bottom of the slide. Average GP value is 0.228±0.088. C) Same as B) but 5 µm above the slide surface. Average GP of 0.269±0.176 with areas of GP of 0.452±0.124. D) Sedimented material from the 50 mol% pre-mixed lipids without enzyme. Average GP of −0.044±0.079. Fields are 19 µm×19 µm.