Figure 12. Effect of product on relative enzyme specific activity and on vesicle size.

A) Specific activity of SMD on C₁₂SM LUVs with increasing molar fractions of C₁₂Cer-1-P. With the exception of pure C₁₂SM and the 50 mol% mixture, all other differences are statistically significant (P<0.003). B) Mean diameter in nm versus time obtained by DLS of C₁₂SM∶C₁₂Cer-1-P LUVs: The black open circles represent pure C₁₂SM untreated vesicles, the green triangles represent untreated C₁₂SM∶C₁₂Cer-1-P vesicles (23 mol%), the closed red squares are C₁₂SM∶C₁₂Cer-1-P vesicles (50 mol%) treated with 1 µg/ml SMD and the open red squares are the same but without enzyme.